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Thioester-mediated biocatalytic amide bond synthesis with in situ thiol recycling - Nature Catalysis

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Thioester-mediated biocatalytic amide bond synthesis with in situ thiol recycling - Nature Catalysis

The activation of carboxylic acids to thioesters plays an important role in biology. However, biochemical studies and biotechnological applications are hampered by a general lack of access to thioesters, especially those based on Coenzyme A (CoA-SH). Here we show a generic thioester recycling enzyme by exploiting the promiscuous activity of a carboxylic acid reductase (CARsr).

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Thioester-mediated biocatalytic amide bond synthesis with in situ thiol recycling - Nature Catalysis

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Thioester-mediated biocatalytic amide bond synthesis with in situ thiol recycling - Nature Catalysis

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