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Purification, full-length sequencing and genomic origin mapping of eccDNA - Nature Protocols

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Purification, full-length sequencing and genomic origin mapping of eccDNA - Nature Protocols

Extrachromosomal circular DNA (eccDNA) was discovered more than half a century ago. However, its biogenesis and function have just begun to be elucidated. One hurdle that has prevented our understanding of eccDNA is the difficulty in obtaining pure eccDNA from cells. The current eccDNA purification methods mainly rely on depleting linear DNAs by exonuclease digestion after obtaining crude circles by alkaline lysis.

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Purification, full-length sequencing and genomic origin mapping of eccDNA - Nature Protocols

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Purification, full-length sequencing and genomic origin mapping of eccDNA - Nature Protocols

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