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Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs) - Nature Protocols

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Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs) - Nature Protocols

Prime editors (PEs), which can install desired base edits without donor DNA or double-strand breaks, have been used in plants and can, in principle, accelerate crop improvement and breeding. However, their editing efficiency in plants is generally low. Optimizing the prime editing guide RNA (pegRNA) by designing the sequence on the basis of melting temperature, using dual-pegRNAs and engineering PEs have all been shown to enhance PE efficiency.

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Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs) - Nature Protocols

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Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs) - Nature Protocols

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