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m6A-SAC-seq for quantitative whole transcriptome m6A profiling - Nature Protocols

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m6A-SAC-seq for quantitative whole transcriptome m6A profiling - Nature Protocols

N6-methyladenosine (m6A) is the most abundant mRNA modification in mammalian cells, regulating many physiological processes. Here we describe a method for base-resolution, quantitative m6A sequencing in the whole transcriptome. The enzyme and small-molecule cofactor used in this protocol are prepared by recombinant protein expression and organic synthesis, respectively.

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m6A-SAC-seq for quantitative whole transcriptome m6A profiling - Nature Protocols

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m6A-SAC-seq for quantitative whole transcriptome m6A profiling - Nature Protocols

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