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An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts - Nature Biotechnology

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An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts - Nature Biotechnology

In vitro transcription (IVT) is a DNA-templated process for synthesizing long RNA transcripts, including messenger RNA (mRNA). For many research and commercial applications, IVT of mRNA is typically performed using bacteriophage T7 RNA polymerase (T7 RNAP) owing to its ability to produce full-length RNA transcripts with high fidelity; however, T7 RNAP can also produce immunostimulatory byproducts such as double-stranded RNA that can affect protein expression.

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An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts - Nature Biotechnology

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An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts - Nature Biotechnology

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