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Spatial mapping of the total transcriptome by in situ polyadenylation - Nature Biotechnology

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Spatial mapping of the total transcriptome by in situ polyadenylation - Nature Biotechnology

Spatial transcriptomics reveals the spatial context of gene expression, but current methods are limited to assaying polyadenylated (A-tailed) RNA transcripts. Here we demonstrate that enzymatic in situ polyadenylation of RNA enables detection of the full spectrum of RNAs, expanding the scope of sequencing-based spatial transcriptomics to the total transcriptome.

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Spatial mapping of the total transcriptome by in situ polyadenylation - Nature Biotechnology

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Spatial mapping of the total transcriptome by in situ polyadenylation - Nature Biotechnology

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