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Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution - Nature Biotechnology

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Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution - Nature Biotechnology

Functional characterization of pseudouridine (Ψ) in mammalian mRNA has been hampered by the lack of a quantitative method that maps Ψ in the whole transcriptome. We report bisulfite-induced deletion sequencing (BID-seq), which uses a bisulfite-mediated reaction to convert pseudouridine stoichiometrically into deletion upon reverse transcription without cytosine deamination.

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Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution - Nature Biotechnology

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Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution - Nature Biotechnology

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