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CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states - Nature Neuroscience

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CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states - Nature Neuroscience

Astrocytes become reactive in response to insults to the central nervous system by adopting context-specific cellular signatures and outputs, but a systematic understanding of the underlying molecular mechanisms is lacking. In this study, we developed CRISPR interference screening in human induced pluripotent stem cell-derived astrocytes coupled to single-cell transcriptomics to systematically interrogate cytokine-induced inflammatory astrocyte reactivity.

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CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states - Nature Neuroscience

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CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states - Nature Neuroscience

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