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Probing coenzyme A homeostasis with semisynthetic biosensors - Nature Chemical Biology

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Probing coenzyme A homeostasis with semisynthetic biosensors - Nature Chemical Biology

Coenzyme A (CoA) is one of the central cofactors of metabolism, yet a method for measuring its concentration in living cells is missing. Here we introduce the first biosensor for measuring CoA levels in different organelles of mammalian cells. The semisynthetic biosensor is generated through the specific labeling of an engineered GFP–HaloTag fusion protein with a fluorescent ligand.

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Probing coenzyme A homeostasis with semisynthetic biosensors - Nature Chemical Biology

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Probing coenzyme A homeostasis with semisynthetic biosensors - Nature Chemical Biology

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