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Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model - Nature Communications

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Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model - Nature Communications

The hindered diffusion model postulates that the movement of a signaling molecule through an embryo is affected by tissue geometry and binding-mediated hindrance, but these effects have not been directly demonstrated in vivo. Here, we visualize extracellular movement and binding of individual molecules of the activator-inhibitor signaling pair Nodal and Lefty in live developing zebrafish embryos using reflected light-sheet microscopy.

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Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model - Nature Communications

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Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model - Nature Communications

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