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NMR-guided directed evolution - Nature

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Directed evolution is a powerful tool for improving existing properties and imparting completely new functionalities to proteins1–4. Nonetheless, its potential in even small proteins is inherently limited by the astronomical number of possible amino acid sequences. Sampling the complete sequence space of a 100-residue protein would require testing of 20100 combinations, which is beyond any existing experimental approach.

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NMR-guided directed evolution - Nature

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NMR-guided directed evolution - Nature

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