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Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation - Nature Methods

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Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation - Nature Methods

Whereas techniques to map chromatin-bound proteins are well developed, mapping chromatin-associated RNAs remains a challenge. Here, we describe Reverse Transcribe and Tagment (RT&Tag), in which RNAs associated with a chromatin epitope are targeted by an antibody followed by a protein A-Tn5 transposome. Localized reverse transcription generates RNA/cDNA hybrids that are subsequently tagmented by Tn5 transposases for downstream sequencing.

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Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation - Nature Methods

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Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation - Nature Methods

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