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Inducible expression of large gRNA arrays for multiplexed CRISPRai applications - Nature Communications

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Inducible expression of large gRNA arrays for multiplexed CRISPRai applications - Nature Communications

CRISPR gene activation and inhibition (CRISPRai) has become a powerful synthetic tool for influencing the expression of native genes for foundational studies, cellular reprograming, and metabolic engineering. Here we develop a method for near leak-free, inducible expression of a polycistronic array containing up to 24 gRNAs from two orthogonal CRISPR/Cas systems to increase CRISPRai multiplexing capacity and target gene flexibility.

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Inducible expression of large gRNA arrays for multiplexed CRISPRai applications - Nature Communications

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Inducible expression of large gRNA arrays for multiplexed CRISPRai applications - Nature Communications

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