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Recording gene expression order in DNA by CRISPR addition of retron barcodes - Nature

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Recording gene expression order in DNA by CRISPR addition of retron barcodes - Nature

Biological processes depend on the differential expression of genes over time, but methods to make physical recordings of these processes are limited. Here we report a molecular system for making time-ordered recordings of transcriptional events into living genomes. We do this through engineered RNA barcodes, based on prokaryotic retrons1, that are reverse transcribed into DNA and integrated into the genome using the CRISPR–Cas system2.

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Recording gene expression order in DNA by CRISPR addition of retron barcodes - Nature

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Recording gene expression order in DNA by CRISPR addition of retron barcodes - Nature

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