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A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide - Scientific Reports

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A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide - Scientific Reports

The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in molecular biology, including transformation of large constructs, chromosomal genetic manipulation, and dense mutant library construction.

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A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide - Scientific Reports

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A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide - Scientific Reports

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