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Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing - Nature Microbiology

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Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing - Nature Microbiology

CRISPR-Cas13 proteins are RNA-guided RNA nucleases that defend against incoming RNA and DNA phages by binding to complementary target phage transcripts followed by general, non-specific RNA degradation. Here we analysed the defensive capabilities of LbuCas13a from Leptotrichia buccalis and found it to have robust antiviral activity unaffected by target phage gene essentiality, gene expression timing or target sequence location. Furthermore, we

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phage editing genome cas13a broad science biology genetics

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Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing - Nature Microbiology

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Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing - Nature Microbiology

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